Composite

Part:BBa_K339009:Design

Designed by: Christopher Tang   Group: iGEM10_Calgary   (2010-10-25)


CpxR Promoter with RFP Reporter and Arabinose Inducible MalE31


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2603
    Illegal BamHI site found at 2076
    Illegal BamHI site found at 2339
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
    Illegal AgeI site found at 1911
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2301
    Illegal SapI site found at 1893


Design Notes

malE31 toxic to cells grown at 3°C,


Source

E. coli

References